| Japanese |
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| Title | 血中Digoxin濃度のRadioimmunoassayによる測定 |
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| Subtitle | 原著 |
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| Authors | 久保田治代*, 黒崎浩巳**, 開原成允*, 飯尾正宏*, 村尾覚*, 太田昭夫*** |
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| Organization | *東大第2内科, **第一ラジオアイソトープ研究所, ***心臓血管研究所 |
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| Journal | 核医学 |
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| Volume | 9 |
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| Number | 3 |
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| Page | 255-263 |
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| Year/Month | 1972/6 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「緒言」1960年BersonとYalowがradioimmunoassayをインシュリンの測定に導入して以来各種の蛋白性ホルモンの定量に著しい効果を発揮してきた. 最近では抗体作製法の進歩によってradioimmunoassayによって測定し得る物質はpolypeptideやsteroidにまで及ぶようになった. 1967年Butler等はdigoxinに対する抗体の産生に成功し, radioimmunoassayによる血清中digoxin濃度の測定を可能にした. 次いで, 1969年Smith等によってこの方法が確立された. 著者等は, これらの報告をもとにして抗体を作製し血清digoxin濃度のradioimmunoassayによる測定を試み, 測定法について改良を加え臨床的に充分用い得るとの結論を得たのでその結果を発表する. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Radioimmunoassay of Serum Digoxin |
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| Authors | Haruyo KUBOTA, Shigekoto KAIHARA*, Masahiro IIO*, Satoru MURAO*, Hiromi KUROSAKI**, Akio OHTA*** |
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| Organization | *The Second Internal Medicine, Uuiversity of Tokyo, **Daiichi Rodiosotope Laboratories, ***Cardiovascular Institute |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 9 |
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| Number | 3 |
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| Page | 255-263 |
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| Year/Month | 1972/6 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | [Summary] A rapid, sensitive method for measuring a plasma digoxin concentration has been developed by the redioimmunoassay technique. Digoxin-BSA (bovine serum albumin) conjugate was prepared by a technique originally described by Butler and Chen. Ten rabbits were immunized by the injection of Digoxin-BSA conjugate with six injections in the foot pads over a 60 day period. The assay was performed by incubation in small test tubes, to which 0.2 ml of sample or standard serum, 0.1 ml of anti-serum and 0.6 ml of of buffer solution. Then tubes were shaken and stood at room temperature for 5min. after that 0.1 ml of 3H-digoxin was added, and incubated again at room temperature for 15 min. Separation of bound from free labeled digoxin was achieved by dextran coated charcoal. The Supernatant phase was added to 15 ml of liquid scincillator which consisted of toluene and Triton X-100 and counted in a liquid scintillation counter. Correction for quenching was made by automatic external standardization. A standard curve was constructed by use of solutions of known concentrations of standard digoxin. The precision of the assay showed that for serum levels in the range 0.0〜0.5 mμg/ml the relative standard deviation was 8.8%, in the range 0.5〜1.5 mμg/ml was 3.2%, and in the range of 1.5〜3.5 mμg/ml was 4.4%. A similar analysis was performed on pooled serum by repeated measurement. The relative standard deviation was 1.145+-0.042 mμg/ml. The results of assays performed upon clinical cases who were administered various doses of digoxin showed digoxin levels in the range 0.0〜4.5 mμg/ml. There is positive correlation between maintenance dosage and plasma digoxin concentration. |
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| Practice | Clinical medicine |
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