| Japanese |
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| Title | Phadebas(R) Insulin Test (Solid Phase Radioimmunoassay) の使用経験 |
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| Subtitle | 原著 |
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| Authors | 稲田満夫, 岡部純一, 風間善雄, 高山英世, 春名桃江, 高橋浩 |
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| Organization | 天理よろず相談所病院内分泌内科 臨床病理部 |
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| Journal | 核医学 |
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| Volume | 8 |
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| Number | 5 |
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| Page | 305-310 |
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| Year/Month | 1971/10 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「はじめに」Radioimmnoassayによる血中ホルモンの測定には二抗体法が広く用いられているが, 更に操作を簡便化し, 測定所要時間を短縮して, 日常検査法として利用しやすくしようとする努力がなされて来た. 最近非常に簡便な抗原抗体複合物の分離法として固相法 (Solid Phase Radioimmunoassay) が注目されている. 著者らは第一ラジオアイソトープ研究所より血中Insulin測定法に固相法を用いたPhadebas(R) Insulin Test (Pharmacia社製) の提供をうけたので, 本稿ではその若干の使用経験について述べる. 「Phadebas(R) Insulin Testの原理」本法ではinsulin抗体を予め化学反応によりsephadexに結合させてある. 従ってinsulin抗体はすでに大きな分子となっているため, それと血中insulin及び標準insulinと反応させると, 結合型insulinは容易に遊離型insulinと分離する事が出来る. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Determinations of insulin concentrations in plasma by solid phase radioimmunoassay (Phadebas(R) Insulin Test) . |
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| Authors | Mitsuo Inada, Junichi Okabe, Yoshio Kazama, Hideo Takayama, Momoe Haruna, Hiroshi Takahashi |
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| Authors(kana) | |
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| Organization | Endocrine Section, Department of Internal Medicine, and Department of Clinical Pathology, Tenri Hospital |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 8 |
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| Number | 5 |
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| Page | 305-310 |
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| Year/Month | 1971/10 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | 1) Determinations were made of insulin concentrations in plasma by Phadebas(R) insulin test. In this new solid phase method of radioimmunoassay for insulin, insulin antibodies were covalently coupled to Sephadex as the solid phase support, and insulin bound to Sephadex anti-insulin complex was easily precipitated to separate from free insulin. 2) After incubation of plasma or insulin standard with 125I-insulin and with Sephadex anti-insulin complex, bound insulin was precipitated by centifugation, and was washed with 0.9% saline solution. 125I activities of washed precipitates were assayed by well type scintillation counter. The ratios of 125I activities of precipitates in standard and plasma sample to that in sample without addition of insulin standard (zero sample) were calculated to construct the standard curves and to determine the insulin concentrations in plasma. 3) The effect of incubation time on insulin concentration in plasma was investigate from comparison of insulin concentrations determined after 3, 5, 16 and 24 hours incubation at room temperature. Insulin concentrations in plasma were clearly elevated with prolongation of incubation time for 3 to 16 hours and it was reached at plateau in incubation of over 16 hours. The results indicated that incubation time of over 16 hours was necessary to obtain accurate insulin concentrations in Plasma. 4) The ratios of activity of bound 125I-insulin to total 125I activity at zero sample were 20.7 +- 6.2%. 25.5 +- 2.4%, and 28.3 +- 3.9% in incubation time of 3, 16 and 24 hours, respectively, at room temperature. The highest ratio was obtained at incubation time of 24 hours, indicating this condition was most suitable to determine insulin concentrations in plasma by the test. 5) Striking correlation was evident in the plots of insulin concentrations in plasma by Phadebas(R) insulin test and those by double antibody method. The findings represent Phadebas(R) insulin test was useful to determine the insulin concentrations in plasma as routine test. |
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| Practice | Clinical medicine |
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