| Japanese |
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| Title | Tetrasorb-125による血中Thyroxine量の測定に関する基礎的ならびに臨床的研究 |
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| Subtitle | 原著 |
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| Authors | 森田陸司, 鳥塚莞爾, 浜本研, 中川毅, 小西淳二, 日下部恒輔 |
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| Organization | 京都大学中央放射線部 |
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| Journal | 核医学 |
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| Volume | 7 |
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| Number | 3 |
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| Page | 180-188 |
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| Year/Month | 1970/8 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「I. はじめに」血中甲状腺ホルモン測定法には, 従来PBI法が用いられており, 最近は遊離型thyroxineおよびtriiodothyronineの測定なども試みられているが, いずれも操作が煩雑かつ熟練を要するため, 一般には敬遠される傾向がある. 一方これに対して, thyroxine (T4) に特異的に結合する蛋白を利用して, これに標識T4と非標識T4を競合させることにより非標識T4量を測定するcompetitive protein binding analysisによるT1測定法がある. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | Fundamental and Clinical Studies on Determination of Serum Thyroxine by Tetrasorb-125 |
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| Authors | Rikushi Morita, Kanji Torizuka, Ken Hamamoto, Tsuyoshi Nakagawa, Junji Konishi, Tsunesuke Kusakabe |
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| Authors(kana) | |
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| Organization | Central Clinical Radioisotope Division and 2nd Division of Internal Medicine, Kyoto University School of Medicine |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 7 |
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| Number | 3 |
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| Page | 180-188 |
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| Year/Month | 1970/8 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | Basic and clinical evaluation for Tetrasorb-125, a diagnostic kit for determination of serum thyroxine by competitive protein binding analysis, were studied and the following results were obtained. 1) Extraction ratios of thyroxine from serum were 80.4% by 95% ethanol and 96.7% by 99.5% ethanol, and the loss of thyroxine in evaporation was negligible. 2) Temperature and duration of incubation influenced the binding of thyroxine to the resin significantly, and it was considered that the incubation at 2-4℃ for 60min. was the most stable and practical. 3) The slope of standard curve using diluted 125I-TBG solution by barbital buffer (pH 8.6) was greater than that with original TBG-solution, and it was found that in measuring thyroxine amount less than 10μg/100ml in its concentration, the use of diluted TBG solution was better in accuracy and reproducibility than that of original TBG solution. 4) Tetrasorb values in normal subjects were ranged from 6 to 13μg/100ml and were differentiated from those with hyperthyroidism and hypothyroidism. In the cases with pregnancy, thyroxine binding globulin deficiency, nephrosis and the cases treated with anabolic steroid, Tetrasorb values were higher or lower, and did not reflect the thyroid function accurately. But in these cases, free thyroxine indices expressed as Triosorb x Tetrasorb / 1-0.6 x Triosorb, which were ranged 2.0-5.0 in euthyroid individuals, had a good correlation with the absolute concentrations of free thyroxine measured by equilibrium dialysis, and reflected the thyroid function accurately. 5) As the standard curve reached plateau over 20μg/100ml of thyroxine, it was necessary to measure a part of alcohol extraction in cases with hyperthyroidism. |
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| Practice | Clinical medicine |
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