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| Title | 中性子放射化分析法による肝の銅・マンガン量の測定 - Wilson病・ヘモクロマトージス・肝硬変について - |
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| Subtitle | 原著 |
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| Authors | 上田英雄*, 岩瀬透*, 飯尾正宏*, 亀田治男*, 長尾博之**, 谷彰** |
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| Organization | *東京大学 上田内科, **日本原子力事業 NAIG総合研究所 |
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| Journal | 核医学 |
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| Volume | 5 |
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| Number | 3 |
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| Page | 157-164 |
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| Year/Month | 1968/10 |
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| Article | 原著 |
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| Publisher | 日本核医学会 |
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| Abstract | 「I. 緒言」微量金属は, 諸種疾患の病態に関与しうる. Wilson病における銅代謝異常, ヘモクロマトージスにおける鉄代謝異常は, その代表的なものである. 従来各種臓器の微量金属含有量測定は, 主として比色法あるいは発光分光分析法のによってきたが, 近年放射化学の発展によりこの分野にも放射化分析法が応用されるようになった. 著者らは, 各種代謝の中心的役割を演ずる肝について, 組織中の微量元素を中性子放射化分析法により定量している. 本論文では銅とマンガンに焦点をしぼり, 剖検あるいは針生検肝試料についての定量法の詳細と, Wilson病・ヘモクロマトージス・肝硬変・正常対照の肝試料についての分析結果ならびにその意義について述べる. 「II. 方法」Wilson病10例(剖検例8例・針生検例2例), ヘモクロマトージス5例(剖検例), 肝硬変13例(剖検例), 対照8例(剖検例6例・針生検例2例)を対象とした. |
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| Practice | 臨床医学：一般 |
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| English |
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| Title | The Determination of Copper and Manganese in the Human Liver by Neutron-Activation Analysis |
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| Authors | Hideo Ueda*, Tohru Iwase*, Masahiro Iio*, Haruo Kameda*, Hiroyuki Nagao**, Akira Tani** |
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| Organization | *The Second Department of Internal Medicine Faculty of Medicine, University of Tokyo, **NAIC Nuclear Research Laboratory |
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| Journal | The Japanese Journal of nuclear medicine |
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| Volume | 5 |
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| Number | 3 |
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| Page | 157-164 |
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| Year/Month | 1968/10 |
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| Article | Original article |
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| Publisher | THE JAPANESE SOCIETY OF NUCLEAR MEDICINE |
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| Abstract | Neutron-activation analysis of copper and manganese was performed both by nondestructive (Cu-66) and chemical separation (Cu-64 and Mn-56) methods on biopsy and autopsy liver samples obtained from 10 cases of Wilson's disease, 5 cases of hemochromatosis and 8 normal cases. Liver samples (1〜100mg dry weight) were irradiated for 10 min.〜4hrs. using TTR-1 100KW swimming pool reactor with a thermal neutron flux of 3×1011n/cm2sec. In chemical separation method, copper was separated with almost 100% yield by internal electro-deposition technique, and then manganese with 90% yield by usual precipitation method. The separation process for copper was completed within 30 min., and for manganese 60 min. Comparison between destructive and chemical separation methods for copper analysis showed well correlation in Wilson's autopsy liver specimens stored in formalin. However, fresh liver specimens obtained by needle biopsy could be analysed only by chemical separation method, because of the presence of plenty amount of sodium. The hepatic copper content was found to be abnormally increased in Wilson's disease (88〜720μg/g dry weight) and also in hemochromatosis (100〜2500μg/g), normal range being 2〜58μg/g. The amount of hepatic manganese in hemochromatosis was 4.1〜17μg/g dry weight, showing significant increase compared with normal range of 1.1〜3.4μg/g. In conclusion, activation analysis of trace elements in the human liverp roved to de useful tool for medical research and diagnosis. By the combination of rapid chemical separation method with thermal neutron irradiation even the needle biopsy sample (e.g. lmg) could be successfully analysed. In Wilson's disease increased level of hepatic copper and in hemochromatosis significant increase in both copper and manganese were observed. |
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| Practice | Clinical medicine |
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