Japanese
Title放射性鉄59Feによる不飽和鉄結合能測定法に関する検討
Subtitle原著
Authors山田英雄
Authors(kana)
Organization名古屋大学日比野内科
Journal核医学
Volume2
Number3/4
Page146-156
Year/Month1965/12
Article原著
Publisher日本核医学会
Abstract「はじめに」不飽和鉄結合能(unsaturated iron-binding capacity, 以下UIBCと略す)の測定法としてはin vivoとin vitroの方法があり, in vitroの方法には直接比色法と間接法がある. 従来広く用いられてきた直接比色法は操作が単純で, 信頼度も高いが, 血清の混濁・溶血・黄疸等による影響が大きく測定が困難な場合のあるほか, 操作の関係上日常臨床検査として同時に多くのサンプルを処理することはむずかしいという欠点がある. 過剰鉄飽和を用いる間接法は種々考案され, これらの欠点を除くことができるといわれているが, これらの方法では鉄の化学的測定を必要とする. RIを利用したUIBC測定法は放射性鉄59Feのこの間接法への導入であり, Feinsteinはすでに1952年59FeCl3を含む鉄規準液を血清に加え, 硫安で除たんぱくし, 濾液と沈澱との放射活性を測定しUIBC値を求める方法を発表している.
Practice臨床医学：一般
Keywords
English
TitleStudies on the Radioisotopic Determination of the Unsaturated Iron-Binding Capacity of Serum
Subtitle
AuthorsHideo Yamada
Authors(kana)
Organization1st Department of Internal Medicine, Nagoya University, School of Medicine
JournalThe Japanese Journal of nuclear medicine
Volume2
Number3/4
Page146-156
Year/Month1965/12
ArticleOriginal article
PublisherTHE JAPANESE SOCIETY OF NUCLEAR MEDICINE
Abstract[Summary] Tauxe's method with minor modification has been employed for UIBC determination in our clinic since 1962. Clinical data of UIBC determinations obtained by this method and fundamental studies performed for validating this methd were reported in this paper. Following results were obtained from studies on method. The amount of resin (Amberlite IRA 410) should be more than 1.5 gram. This amount of resin can give more than 97.6% of removal rate. Magnesium carbonate is available instead of resin for removing excess free iron. Removal rate by magnesium carbonate is higher than that of resin. Spiking solution of radioactive feerric ammonium citrate keeps its stability for several months when stored in a 4℃ refrigerator. For incubation period, 10 minutes incubation can give the same values as longer incubation except for highly saturated sera. Prolonged incubation should be avoided for highly saturated sera, for non specific binding occurs. Hemolysis and jaundice do not interfere with the measurement of UIBC by this method. Serum storage at 4℃ can give reproducible results for at least 10 days. Summary of UIBC values measured by this radioactive method is as follows. It has been shown, based on these clinical and experimental results, that this radioisotopic method is admirably excellent and easily adaptable as a routin laboratory test.
PracticeClinical medicine
Keywords

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